G - Physics – 01 – N
Patent
G - Physics
01
N
150/11
G01N 33/48 (2006.01) C12Q 1/56 (2006.01) G01N 31/02 (2006.01)
Patent
CA 1052676
ABSTRACT OF THE DISCLOSURE There is not known a rapid test for the determination of procoagul- ants in the blood. The presence of procoagulants is an indication of thrombos- is or a prethrombosis condition. The present invention overcomes this draw- back by providing a method of measuring serum coagulability in a patient com- prising the steps of adding heparin in different amounts to aliquots of serum from a patient, adding each of the resultant heparin-serum solutions to a separate tube containing thrombin solution, reacting a portion of each of the resultant thrombin-heparin-serum solutions with normal plasma solution to determine whether clotting occurs, and in the event clotting occurs, deter- mining the clotting time, all steps being carried out under standardized conditions, thereby making it possible to compare the measured clotting times with clotting time ranges obtained under similar conditions with serum from healthy unmedicated individuals and from known thrombotic individuals, and to establish whether the measured clotting times fall within a first normal range corresponding to normal, unmedicated individuals, a second low range corresponding to thrombotic individuals, or third and fourth ranges intermedi- ate said normal and low ranges and corresponding to individuals of increasing coagulability, a low clotting time correlating with high serum coagulability.
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