Method for detection of virulent strain of influenza type-a...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/10 (2006.01) G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) C07K 14/11 (2006.01)

Patent

CA 2634968

[PROBLEMS] To provide a method for detecting a virulent strain of influenza type-A virus in a specific, rapid and simple manner and a determination apparatus. [MEANS FOR SOLVING PROBLEMS] A method for detection of a virulent strain of influenza type-A virus, which uses a first antibody responsible to any subtype of influenza type-A virus and a second antibody non-responsible to a virulent strain of influenza type-A virus but responsible to any subtype of influenza type-A virus other than the virulent strain and comprises the step of detecting an antigen that shows a positive response against the first antibody and a negative response against the second antibody by an immunoassay.

L'invention vise un procédé destiné à la détection simple et rapide d'une souche virulente du virus grippal A ainsi qu'un appareil correspondant. Selon l'invention, un procédé de détection d'une souche virulente du virus grippal A est caractérisé en ce qu'il utilise un premier anticorps réagissant à n'importe quel sous-type du virus grippal A et un deuxième anticorps non réagissant à une souche virulente du virus grippal A mais réagissant à n'importe quel sous-type du virus grippal A autre que la souche virulente. Le procédé consiste à détecter un antigène qui manifeste une réponse positive vis-à-vis du premier anticorps et une réponse négative vis-à-vis du deuxième anticorps au moyen d'un immunodosage.

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