C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2748117
The present invention relates to the identification of Xenotropic murine leukemia virus (XMRV) nucleic acid by polymerase chain reaction (PCR) analysis (e.g., real time PCR (RT/PCR); nested RT/PCR using Tth DNA polymerase and Hot start polymerase) and the uses thereof. In particular, the invention provides methods for the detection, and in particular early detection, of XMRV in RNA isolated from samples (e.g., urine samples; expressed prostate secretion (EPS)) of prostate cancer patients and normal individuals.
La présente invention concerne l'identification de l'acide nucléique du virus de la leucémie murine xénotropique (XMRV) par analyse de l'amplification en chaîne par polymérase (PCR) (par exemple PCR en temps réel (RT/PCR); RT/PCR niché utilisant l'ADN polymérase Tth et la polymérase « Hot start »), ainsi que des utilisations afférentes. L'invention concerne en particulier des procédés de détection, et plus spécifiquement de détection précoce, du XMRV dans un ARN isolé sur des échantillons (par exemple échantillons d'urine, sécrétion de la prostate exprimée (EPS)) de patients atteints du cancer de la prostate et d'individus normaux.
Gupta Jaydip Das
Klein Eric A.
Nguyen Carvell T.
Silverman Robert H.
Weight Christopher J.
Gowling Lafleur Henderson Llp
The Cleveland Clinic Foundation
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