Method for detection or determination if environmental hormones

G - Physics – 01 – N

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G01N 33/00 (2006.01) C12Q 1/02 (2006.01) G01N 31/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2397637

A method of detecting or quantifying environmental hormones (in particular, dioxins) in a sample with the use of granulosa cells, etc. In this method, changes in the expression dose of a luteinizing hormone receptor induced by follicle-stimulating hormone, etc. in the granulosa cells are employed as an indication for detecting or quantifying dioxins. A contraceptive method characterized by inhibiting the expression of luteinizing hormone receptor induced by follicle-stimulating hormone in granulosa cells; and contraceptive agents containing a substance inhibiting the expression of luteinizing hormone receptor as the active ingredient.

La présente invention concerne la recherche ou le dosage de perturbateurs endocriniens, et notamment les dioxines, dans des prélèvements, essentiellement par utilisation des cellules de la granulosa. Ce procédé consiste à considérer les variations de la dose d'expression d'un récepteur d'hormone lutéinisante induites notamment par l'hormone folliculostimulante dans les cellules de la granulosa et à les utiliser comme indication pour la recherche ou le dosage des dioxines. L'invention concerne également un procédé contraceptif dont la caractéristique est qu'il inhibe l'expression du récepteur d'hormone lutéinisante dans les cellules de la granulosa. L'invention concerne enfin des agents contraceptifs dont le principe actif est une substance inhibitrice de l'expression du récepteur d'hormone lutéinisante.

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