G - Physics – 01 – N
Patent
G - Physics
01
N
167/39, 167/40,
G01N 33/573 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01) G01N 33/60 (2006.01) G01N 33/66 (2006.01) G01N 33/80 (2006.01)
Patent
CA 2012062
A method for the determination of glycosyl- transferase activities as well as its use in blood grouping and the diagnosis of cancer are disclosed. In one aspect of the method, an oligosaccharide linked to an insoluble carrier is used as a sugar acceptor to obtain an immobilized reaction product, an endogenous product of the same structure as the reaction product in a specimen is rinsed out and then the amount of the reaction product is measured. In the other aspect of the method, an oligosaccharide linked to a protein is used as a sugar acceptor to obtain a reaction product, after immobilization of the reaction product with an insoluble antibody recognizing the protein portion thereof, the endogenous product is rinsed out and the reaction product is sandwiched with another antibody recognizing the sugar portion thereof to determined glycosyltransferase by measuring of the amount of the reaction product.
Kirby Eades Gale Baker
Otsuka Pharmaceutical Co. Ltd.
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