C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.1
C12Q 1/58 (2006.01) C12N 9/06 (2006.01) C12Q 1/26 (2006.01) C12Q 1/32 (2006.01)
Patent
CA 1229781
ABSTRACT OF THE DISCLOSURE The quantity of ammonia in a sample to be measur- ed for ammonia content can be determined by: (A) contacting the sample with L-glutamate dehydrogenase in the presence of reduced nicotinamide- adenine dinucleotide or reduced nicotinamide-adenine dinucleotide phosphate and .alpha.-ketoglutarate to produce glutamate in a quantity corresponding to the ammonia content in the sample; (B) catalyzing the resultant mixture by L-glutamate oxidase in the presence of oxygen to produce hydrogen peroxide in a quantity corresponding to the glutamate content in the resultant mixture; (C) measuring the quantity of hydrogen peroxide produced or the production rate thereof; and (D) calculating the ammonia content from the quantity of hydrogen peroxide produced or the produc- tion rate thereof thus measured. In accordance with the method of the present inven- tion described above, it has become possible to determine the quantity of ammonia with high sensitivity in a short period of time by using as a signal a measura- ble value associated with hydrogen peroxide via a con- tinuous autoanalyzing system.
460754
Murachi Takashi
Tabata Masayoshi
Kenkyusho Rikagaku
Macrae & Co.
Yamasa Shoyu Kabushiki Kaisha
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