C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/60 (2006.01) G01N 33/92 (2006.01)
Patent
CA 2190282
The present invention relates to a method for the determination of cholesterol in low-density lipoprotein (LDL) in a sample containing LDL, which comprises eliminating cholesterol in high-density lipoprotein in the sample, subjecting the sample to a reaction utilizing the action of a cholesterol ester-hydrolyzing enzyme and the action of a cholesterol-oxidizing enzyme or of cholesterol oxidoreductase, and determining the amount of hydrogen peroxide ora reduced type coenzyme generated by the reaction.
L'invention concerne un procédé pour l'évaluation quantitative du cholestérol dans un lipoprotéine faible densité, qui consiste à éliminer le cholestérol d'une lipoprotéine haute densité dans un échantillon porteur de lipoprotéine à faible densité, à traiter ensuite l'échantillon résultant par cholestérol ester hydrolase et par cholestérol oxydase ou oxydoréductase et, enfin, à évaluer quantitativement le peroxyde d'hydrogène obtenu ou bien la coenzyme réduite.
Miike Akira
Miyauchi Kazuhito
Goudreau Gage Dubuc
Kyowa Medex Co. Ltd.
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