Method for determination of creatine phosphokinase activity...

Details Method for determination of creatine phosphokinase activity... Method for determination of creatine phosphokinase activity...

G - Physics

01

N

150/15.5

G01N 33/00 (2006.01) C12Q 1/50 (2006.01)

Patent

CA 1062592

ABSTRACT An improved method and compositions for the determination of creatine phosphokinase in a body fluid, which method comprises (a) incubating the body fluid with a thiol activator, creatine phosphate, hexokinase, glucose-6- -phosphate dehydrogenase, glucose, nicotinamide adenine di- nucleotide, and from 0.02 to 0.10% weight/volume of a non- ionic ether-ester polyoxyethylated vegetable oil surfactant for a time sufficient to activate the creatine phosphokinase; (b) mixing the resulting incubate with adenosine-5'-diphosphate, the tetrazolium salt dye, and adenosine-5'-monophosphate; (c) incubating the resulting mixture for a time sufficient to develop measurable color; and (d) stopping the reaction after a pre-determined time with an acid.

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