C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/54 (2006.01) G01N 21/03 (2006.01) G01N 21/31 (2006.01) G01N 33/66 (2006.01) G01N 15/00 (2006.01) G01N 21/78 (2006.01) G01N 33/493 (2006.01)
Patent
CA 2053284
A sample of whole blood is contacted with a reagent which by chemical reaction with glu- cose in the sample brings about a detectable dye concentration change (10) the size of which is de- termined as a measure of the glucose content of the sample. The sample is initially introduced un- diluted in a microcuvette having at least one cavi- ty for receiving the sample. The cavity is internal- ly pretreated with the reagent in dry form, and the chemical reaction takes place in the cavity. Active components of the reagent comprise at least a hemolysing agent for exposing glucose contained in the blood cells of the sample for al- lowing total glucose determination, and agents taking part in tho chemical reaction and ensuring that the dye concentration change (10) takes place at least in a wavelength range (14) outside the absorption range (12) of the blood hemoglob- in. An absorption measurement is performed in said wavelength range directly on the sample in the cuvette. A pretreated disposable cuvette with such a reagent and a photometer are also de- scribed.
Lilja Jan E.
Nilsson Sven-Erik L.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Migrata Uk Ltd.
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