Method for determination of nucleated red blood cells and...

G - Physics – 01 – N

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G01N 15/14 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2673031

A method for enumerating white blood cells and nucleated red blood cells. The method comprises the steps of: (a) providing a lysed sample of whole blood; (b) introducing the lysed sample to a light-scattering multi-angle depolarizing flow cytometer; (c) removing depolarizing interference, e.g., lipid droplets and other measured particles; (d) differentiating nucleated red blood cells and noise from white blood cells in the absence of depolarizing interference; (e) differentiating nucleated red blood cells from noise in the absence of depolarizing interference and white blood cells; and (f) differentiating possible platelet clumps.

L'invention concerne un procédé de numération de globules blancs et de globules rouges nucléés. Le procédé comprend les étapes consistant à : (a) fournir un échantillon lysé de sang total; (b) introduire l'échantillon lysé dans un cytomètre de flux à dépolarisation multi-angle et à diffusion de lumière; (c) l'élimination d'une interférence de dépolarisation, par exemple des gouttelettes de lipide et d'autres particules mesurées; (d) la différentiation des globules rouges nucléés par rapport au bruit des globules blancs en l'absence d'interférence de dépolarisation; (e) la différentiation des globules rouges nucléés par rapport au bruit en l'absence d'interférence de dépolarisation et de globules blancs; et (f) la différentiation d'agglutinations de plaquettes éventuelles.

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