Method for determination of the gross...

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/34 (2006.01) C12Q 1/25 (2006.01) C12Q 1/48 (2006.01) C12Q 1/527 (2006.01)

Patent

CA 2317959

The invention facilitates a determination of the rate at which soil organic nitrogen is converted to inorganic nitrogen or mineral nitrogen. This conversion is known as N-mineralization. The total release of plant available nitrogen in a soil, i.e. the gross N-mineralization rate quantifies the conversion in the soil of organic nitrogen to inorganic nitrogen. The present invention provides a quick and reliable method for measuring the gross N-mineralization rate of a soil sample. The method exploits that a microbial enzyme activity of a functional ornithine acid cycle present in said sample is correlatable with the rate of gross N-mineralization of that same sample. The method of the invention can be used e.g. in a determination of the amount of a nitrogen- containing fertiliser to be applied to a soil such as e.g. a field of agricultural crops.

L'invention permet de faciliter une détermination du taux selon lequel l'azote organique d'un sol est transformé en azote inorganique ou minéral. Cette transformation est connue sous le nom de N-minéralisation. Le dégagement total d'azote de plantes dans un sol, c.-à-d. le taux de N-minéralisation brute détermine la quantité de transformation d'azote organique en azote inorganique dans le sol. La présente invention concerne un procédé rapide et fiable de mesure du taux de N-minéralisation brute d'un échantillon de sol. Le procédé repose sur le fait que l'activité d'une enzyme microbienne d'un cycle fonctionnel d'ornithine présente dans ledit échantillon peut être corrélée au taux de N-minéralisation brute de cet échantillon. Le procédé de l'invention peut être utilisé, p. ex. pour déterminer la quantité d'engrais contenant de l'azote à appliquer à un sol tel que p. ex. un champ de cultures agricoles.

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