C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/14, 150/3.2
C12Q 1/32 (2006.01) C12Q 1/40 (2006.01) C12Q 1/52 (2006.01)
Patent
CA 1136027
ABSTRACT OF THE DISCLOSURE An improvement in a method for determining a substance present in a biological fluid by oxidizing a compound produced in the course of the determination in the presence of dehydrogenase enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the substance in the fluid, which fluid also contains an endogenous material which or derivative of which produced during the determina- tion likewise is oxidized in the presence of the enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide, thereby interfering with the determina- tion, which improvement involves first carrying out the oxidation-reduction reaction of the endogenous material or its derivative, then oxidizing the resulting reduced beta- nicotinamide adenine dinucleotide in the presence of lactate dehydrogenase with the simultaneous reduction of pyruvate to lactate, thereafter inhibiting the lactate dehydrogenase enzymatic activity, and thereafter producing a quantity of the aforesaid compound produced in the course of the determination for conducting the oxidation-reduction reaction therewith without interference caused by the endogenous material or the lactate dehydrogenase. Substances which may be determined include alpha-amylase, transaminases, and triglycerides. A two-reagent combination is employed for the determination.
332499
Bucolo Giovanni
Madappally Mathew M.
Borden Ladner Gervais Llp
Coulter Electronics Inc.
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