Method for determining activated coagulation factors in...

Details Method for determining activated coagulation factors in... Method for determining activated coagulation factors in...

G - Physics

01

N

G01N 33/86 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 2286164

The invention relates to a method for determining activated coagulation factors in plasma or plasma derivatives. A sample of the plasma or plasma derivative to be examined is incubated with an activated prothrombin complex and procoagulative phospholipid vesicles, the latter containing integrated tissue factor, and thrombin formation is initiated by addition of an appropriate amount of Ca ions. After a determined incubation time, thrombin formation is terminated and the amount of thrombin formed is determined by known methods.

L'invention concerne un procédé pour la détermination des facteurs activés de la coagulation sanguine dans le plasma et les dérivés du plasma. Une quantité d'échantillon de plasma ou de dérivé de plasma à examiner est incubée avec un complexe activé de prothrombine et de vésicules phospholipidiques procoagulatrices, ces dernières renfermant un facteur de tissu intégré, et la formation de thrombine est démarrée par addition d'une quantité appropriée d'ions Ca. Après une durée d'incubation déterminée, la formation de thrombine est achevée et la quantité de thrombine formée est déterminée par des procédés connus.

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