Method for determining alkaline phosphatase

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/42 (2006.01)

Patent

CA 2346297

The invention concerns a method for the determination of alkaline phosphatase in a sample by optical measurement which is characterized in that a main measurement wavelength of 450 ~ 10 nm in combination with the rate blank procedure is used to eliminate haemoglobin interference, a method for eliminating interference by free haemoglobin or blood substitutes and the use of the combination of a main measurement wavelength with the rate blank procedure to eliminate interference by free haemoglobin or blood substitutes in the determination of alkaline phosphatase.

L'invention concerne un procédé permettant de détecter la phosphatase alcaline dans un échantillon par mesure optique, qui se caractérise en ce que pour éliminer les troubles induits par hémoglobine, on utilise une longueur d'ondes de mesure principale de l'ordre de 450+/-10 nm en combinaison avec un procédé </= Rate-Blank >/= . L'invention concerne en outre une méthode permettant d'éliminer des troubles induits par l'hémoglobine libre ou des substituts sanguins lors de la détection de phosphatase alcaline.

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