Method for determining antibiotics with .beta.-lactam core...

G - Physics – 01 – N

Patent

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Details

G01N 33/566 (2006.01) G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/569 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2335734

The invention concerns a method for detecting antibiotics with beta -lactam core in a biological fluid comprising the following steps: a) contacting a specific volume of said biological fluid with an amount of identifying agent and incubating the resulting mixture in conditions for forming a complex of the antibiotics possibly present in said biological fluid with the identifying agent; b) contacting the mixture resulting from step a) with at least a reference antibiotic immobilised on a support, in conditions for forming a complex of the reference antibiotic with the amount of identifying agent which has not reacted in step a); and c) determining the amount of identifying agent fixed on the support. The invention is characterised in that the identifying agent comprises a receptor sensitive to antibiotics with beta -lactam core obtained from Bacillus licheniformis.

Procédé pour la détection d'antibiotiques à noyau .beta.-lactame dans un liquide biologique comprenant les étapes suivantes: a) la mise en contact d'un volume déterminé dudit liquide biologique avec une quantité d'agent de reconnaissance et l'incubation du mélange ainsi obtenu dans des conditions permettant la complexation des antibiotiques éventuellement présents dans ledit liquide biologique avec l'agent de reconnaissance; b) la mise en contact du mélange obtenu à l'étape a) avec au moins un antibiotique de référence immobilisé sur un support, dans des conditions permettant la complexation de l'antibiotique de référence avec la quantité d'agent de reconnaissance qui n'a pas réagi à l'étape a); et c) la détermination de la quantité d'agent de reconnaissance fixée sur le support, caractérisé en ce que l'agent de reconnaissance comprend un récepteur sensible aux antibiotiques à noyau .beta.- lactame obtenu à partir de Bacillus licheniformis.

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