Method for determining base sequence of dna or dna fragment...

Details Method for determining base sequence of dna or dna fragment... Method for determining base sequence of dna or dna fragment...

C - Chemistry, Metallurgy

12

Q

150/9, 358/5.06

C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01T 1/29 (2006.01)

Patent

CA 1257184

METHOD FOR DETERMINING BASE SEQUENCE OF DNA OR DNA FRAGMENT UTILIZING AUTORADIOGRAPHY ABSTRACT OF THE DISCLOSURE A method for determining the base sequence of DNA or DNA fragment utilizing autoradiography which comprises steps of: (1) preparing a mixture of radioactively labeled synthetic DNAs which are complementary to the DNA or DNA fragment to be sequenced; (2) resolving said mixture of synthetic DNAs on a support medium; (3) placing said support medium and a stimulable phosphor sheet in layers for a given period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the support medium, exciting said phosphor sheet with an electromagnetic wave to release the radia- tion energy stored in the phosphor sheet as stimulated emission, and detecting the stimulated emission, to obtain locational information on said radioactively labeled synthetic DNAs; and (4) determining the base sequence of said DNA or DNA fragment according to the obtained locational infor- mation.

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