C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2461133
The invention provides a novel, non-destructive and dynamic process for determining the cell cycle position of living cells. The invention also provides DNA constructs, and cell lines containing such constructs, that exhibit activation and deactivation of a detectable reporter molecule in a cell cycle specific manner. The invention thus allows greater precision in determining cell cycle phase status than existing techniques and further provides a method for continuous monitoring of cell cycle progression in individual cells.
La présente invention concerne un nouveau procédé dynamique et non destructif pour déterminer la position de cellules vivantes dans le cycle cellulaire. La présente invention concerne également des constructions d'ADN et des lignées cellulaires contenant de telles constructions, qui peuvent activer et désactiver une molécule rapporteuse pouvant être détectée de manière spécifique au cycle cellulaire. Cette invention permet donc de déterminer l'état de phase du cycle cellulaire avec une plus grande précision que les techniques existantes. En outre, cette invention concerne un procédé pour surveiller de manière continue la progression du cycle cellulaire dans des cellules individuelles.
Francis Michael John
Goodyer Ian David
Ismail Rahman Aziz
Jones Anne Elizabeth
Kendall Jonathan Mark
Amersham Biosciences Uk Limited
Cancer Research Technology Limited
Fetherstonhaugh & Co.
Ge Healthcare Uk Limited
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