Method for determining hiv-1 subtype

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/48 (2006.01) C07K 14/16 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2376609

A method for determining a subtype of HIV-1 characterized by comprising effecting a nucleic acid amplification reaction acid by using, as a target sequence, a part of the nucleotide sequence of HIV-1 env gene, wherein at least one of the 5'-terminal and 3'-terminal nucleotide sequences differs from subtype to subtype of HIV-1, and detecting the subtype depending on the occurrence of the nucleic acid amplification; and a kit for determining a subtype of HIV-1 containing a pair of primers the target sequence of which is a part of the nucleotide sequence of HIV-1 env gene wherein at least one of the 5'-terminal and 3'-terminal nucleotide sequences differs from subtype to subtype of HIV-1.

L'invention porte sur une méthode permettant de déterminer un sous-type du VIH-1 consistant à effectuer une amplification d'acide nucléique en utilisant comme séquence cible une partie de la séquence nucléotidique du gène du VIH-1 eng dont les séquences nucléotidiques 5'-terminal et 3'-terminal diffèrent de sous-type en sous-type du VIH-1, puis à détecter le sous-type en fonction de la survenance de l'amplification d'acide nucléique. L'invention porte également sur une trousse permettant de déterminer un sous-type du VIH-1 contenant une paire d'amorces dont la séquence cible fait partie de la séquence nucléotidique du gène du VIH-1 eng, dont les séquences nucléotidiques 5'-terminal et 3'-terminal diffèrent de sous-type en sous-type du VIH-1.

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