G - Physics – 01 – N
Patent
G - Physics
01
N
150/11
G01N 33/92 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01)
Patent
CA 1253778
METHOD FOR DETERMINING LIPID BOUND SIALIC ACID IN PLASMA ABSTRACT OF THE DISCLOSURE The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by a method which may be automated involving the following steps: diluting the sample with distilled water; mixing and then cooling the diluted sample; adding a mixture of a chlorinated lower alkyl hydrocarbon and a lower alkyl alcohol; mixing, dilut- ing with water and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a mixture of a protein precipitating agent and adsorbing material, mixing the resulting admixture; recovering the resulting precipi- tate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present. The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis. By so determining the amount of lipid bound sialic acid present in a plasma sample and comparing the amount with values obtained for subjects known to have cancer, one may diagnose the presence of cancer in a subject. Alterna- tively, by determining the amount of lipid bound sialic acid present in plasma samples obtained from a subject over a period of time and comparing each amount so determined with preceding amounts one may monitor the progression, re- mission, or recurrence of cancer in a subject.
477565
Dianon Systems Inc.
Gowling Lafleur Henderson Llp
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