G - Physics – 01 – N
Patent
G - Physics
01
N
150/11
G01N 33/92 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01)
Patent
CA 1255199
METHOD FOR DETERMINING LIPID BOUND SIALIC ACID IN WHOLE BLOOD Abstract of the Disclosure The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated, involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable, substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent, pre- ferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and deter- mining the amount of lipid bound sialic acid present. The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis. By so determining the amount of lipid bound sialic acid present in a whole blood sample and comparing the amount with values obtained for subjects known to have cancer one may diagnose the presence of cancer in a subject. Alter- natively, by determining the amount of lipid bound sialic acid present in whole blood samples obtained from a subject over a period of time and comparing each amount so determined With preceding amounts one may monitor the progression, remission or recurrence of cancer in a subject.
477546
Dianon Systems Inc.
Gowling Lafleur Henderson Llp
LandOfFree
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