Method for determining multimers of plasma proteins

Details Method for determining multimers of plasma proteins Method for determining multimers of plasma proteins

G - Physics

01

N

G01N 27/447 (2006.01) G01N 33/53 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2444179

A method for the qualitative and quantitative determination of multimers of fibrinogen and of von Willebrand factor by gel electrophoresis, in which a sample containing von Willebrand factor (vwF) or fibrinogen is fractionated by submarine electrophoresis using a continuous, homogeneous agarose gel free of lumps, and the multimer bands are visualized immunochemically after a Western blot analysis by a specific antibody-enzyme conjugate on the blotting membrane or by a suitable dye, preferably with a blue stain, in the gel, is described.

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