Method for determining protein solubility

Details Method for determining protein solubility Method for determining protein solubility

G - Physics

01

N

G01N 33/68 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2578546

The present invention relates to methods of screening for expression of a soluble candidate protein within an expression library of candidate proteins. The method involves fusing each candidate protein in the library to a peptide substrate and identifying cells that express soluble candidate protein by detecting enzymatic modification of the peptide substrate.

L'invention concerne des procédés de criblage pour cribler l'expression d'une protéine soluble d'intérêt potentiel dans une bibliothèque d'expression de protéines d'intérêt potentiel. Un procédé selon l'invention consiste à faire fusionner chaque protéine d'intérêt potentiel de la bibliothèque avec un substrat peptidique, et à identifier les cellules qui expriment la protéine soluble d'intérêt potentiel pour détecter une modification enzymatique du substrat peptidique.

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