Method for determining the abundance of sequences in a sample

Details Method for determining the abundance of sequences in a sample Method for determining the abundance of sequences in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2574832

The invention relates to a method or determination of the abundance of a given sequence or several sequences identical or nearly identical to the given sequence in a sample. The method comprises the following steps: carrying out one or more amplification reactions by means of which several different sections of the sequence or sequences of the sample are amplified to give an amplified product, detection of whether given different sections of the sequence of the sample have been amplified and determination of the number of the sequence(s) in the sample by means of the abundance of the presence or otherwise of the given different sections in the amplified product.

Procédé permettant de déterminer la fréquence d'une séquence prédéterminée ou de plusieurs séquences identiques ou presque identiques à la séquence prédéterminée dans un échantillon. Ledit procédé consiste à effectuer une ou plusieurs réactions d'amplification grâce auxquelles plusieurs segments différents de la séquence ou des séquences de l'échantillon peuvent être amplifiées pour obtenir un produit d'amplification, à détecter si des segments différents déterminés de la séquence de la sonde ont été amplifiés et à déterminer le nombre de la (des) séquence(s) dans la sonde à l'aide de la fréquence de présence ou d'absence des segments différents déterminés dans le produit d'amplification.

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