Method for determining the amount of conjugated taxane in...

Details Method for determining the amount of conjugated taxane in... Method for determining the amount of conjugated taxane in...

G - Physics

01

N

G01N 33/50 (2006.01)

Patent

CA 2680073

The invention relates to a method for determining the amount of conjugated taxane, in particular paclitaxel, in a PGA-taxane conjugate said method comprising: a) reacting the PGA-taxane conjugate with a compound formula (I): R1R2N-NH2 (I), wherein R1 and R2 are as defined in the description; to give a unbound taxane and a PGA hydrazide and b) determining the amount of unbound taxane.

Cette invention concerne un procédé permettant de déterminer la quantité de taxane conjuguée, plus particulièrement, le paclitaxel, dans un conjugué PGA-taxane. Le procédé décrit dans l'invention consiste: a) à faire réagir le conjugué PGA-taxane avec un composé représenté par la formule (I) R1R2N-NH2 (I), dans cette formule R1 et R2 sont tels que définis dans la description; afin d'obtenir une taxane non liée et une hydrazide PGA et b) à déterminer la quantité de taxane non liée.

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