Method for determining the amount of template nucleic acid...

Details Method for determining the amount of template nucleic acid... Method for determining the amount of template nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2575217

A method for determining the amount of template nucleic acid present in a sample comprising the steps of: i) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification reaction; iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.

L'invention concerne un procédé permettant de déterminer la quantité d'acide nucléique matrice présent dans un échantillon, qui consiste: i) à mettre en association avec l'échantillon tous les constituants nécessaires à l'amplification de l'acide nucléique, et tous les constituants nécessaires à un essai par bioluminescence pour l'amplification de l'acide nucléique, puis ii) à effectuer l'amplification de l'acide nucléique, iii) à surveiller l'intensité de la lumière émise par l'essai par bioluminescence et iv) à déterminer la quantité d'acide nucléique matrice présent dans l'échantillon.

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