G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/50 (2006.01) C12G 1/00 (2006.01)
Patent
CA 2542533
The present invention relates to a method for determining in vitro the global antiradical defense potential of a living organism or a physical, chemical or biological agent. Said method, which comprises the use of free radicals as a means for inducing cellular lysis and the evaluation of said cellular lysis, is characterised in that it comprises hydrolysing a cellular material before or after the release of free radicals from a free-radical generator into the resulting reaction medium. Said method is useful for following-up the health condition of human beings, animals and plants in vitro, managing the stresses thereof and retarding the ageing thereof. The antiradical substances are particularly useful against spongiform encephalitis.
La présente invention a trait à un procédé de détermination in vitro du potentiel de défense antiradicalaire global d~un organisme vivant ou d~un agent physique, chimique ou biologique, ledit procédé, qui comporte l~utilisation de radicaux libres en tant que moyen induisant la lyse cellulaire puis l~évaluation de ladite lyse cellulaire, étant caractérisé en ce qu~il comprend l~hydrolyse d~un matériau cellulaire avant ou pendant la libération, dans le milieu réactionnel résultant, de radicaux libres à partir d~un générateur de radicaux libres. Ce procédé est utile pour le suivi in vitro de l~état de santé de l~homme, des animaux et des plantes, la gestion de leurs stress et le ralentissement de leur vieillissement. Les substances antiradicalaires sont en particulier utiles vis-à-vis de l~encéphalite spongiforme.
Goudreau Gage Dubuc
Prost Michel
LandOfFree
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