Method for determining the concentration of thrombin inhibitors

Details Method for determining the concentration of thrombin inhibitors Method for determining the concentration of thrombin inhibitors

C - Chemistry, Metallurgy

12

Q

C12Q 1/56 (2006.01)

Patent

CA 2361672

The invention relates to a method for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid. It comprises the following steps. The body liquid is taken from a living body, and the body liquid is subjected to a separation from the turbid matter, if necessary. To the non-turbid body liquid thus obtained are added a coagulation-inhibiting substance not interfering in the transformation prothrombin/active meizothrombin or meizothrombin-des fragment 1 resp., a chromogenic or fluorogenic substrate dissociable by active meizothrombin or meizothrombin-des fragment 1, resp., and a substance dissociating prothrombin into meizothrombin or meizothrombin-des fragment 1, resp., and as an option prothrombin. The thus obtained solution or mixture, resp., is subjected to a wavelength-selective light absorption or light emission measurement as a function of the time. From the reduction of the light absorption or light emission per time unit is determined the amount of the thrombin inhibitor included in the body liquid by comparison to previously determined standard curves.

Procédé permettant de déterminer la concentration d'inhibiteurs de thrombine dans un liquide corporel non trouble ou dans un extrait non trouble de liquide corporel. Selon ledit procédé, du liquide corporel est prélevé chez un être vivant et séparé, si nécessaire, des matières troubles. Un anticoagulant n'intervenant pas dans le processus de conversion de la prothrombine en méizothrombine active ou Mtdesfg1, un substrat chromogène ou fluorogène qui peut être clivé par la méizothrombine active ou Mtdesfg1, une substance qui clive la prothrombine en méizothrombine ou Mtdesfg1e et éventuellement de la prothrombine sont ajoutés au liquide non trouble ainsi obtenu. Le mélange ainsi obtenu est soumis à une mesure d'absorption de la lumière ou d'émission de la lumière à sélectivité des longueurs d'ondes en fonction du temps. La quantité de l'inhibiteur de thrombine contenu dans le liquide corporel est déterminée par comparaison avec des courbes standard déterminées sur la base d'une diminution de l'absorption ou de l'émission de lumière.

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