Method for determining the effects of external stimuli on...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2687084

The present invention describes methods for carrying out experiments on living cells, including making measurements of the operation transcriptional regulatory processes and indicators of the kinds of processes operating in the cell in response to external stimuli. Image analysis allows for gathering data concerning the flow of information through a cell's genomic regulatory network as it is executing a programmatic change in its activities as a function of said stimuli. The method also allows collection of data of the results of the information-processing in the cell by observing the decisions the cell makes when modulating cellular process activities.

L'invention concerne des procédés pour effectuer des expériences sur des cellules vivantes, y compris la réalisation de mesures des processus régulateurs transcriptionnels de fonctionnement et des indicateurs des types de processus fonctionnant dans la cellule en réponse aux stimuli externes. L'analyse d'image permet de recueillir des données concernant le flux d'information grâce à un réseau de régulation génomique de cellule pendant qu'elle exécute un changement programmatique dans ses activités en fonction des stimuli. Le procédé permet également la collecte de données des résultats du traitement d'informations dans la cellule grâce à l'observation des décisions prises par la cellule lors de la modulation des activités de processus cellulaire.

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