Method for diagnosing early and late lyme borreliosis

G - Physics – 01 – N

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Details

G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2468332

The present invention relates to a method for detecting Borrelia burgdorferi sensu lato infection or the presence of antibodies against Borrelia species in a body fluid from a suspected infected or vaccinated human or other mammal, to a diagnostic kit useful in said method and to an immunoassay method for diagnosing early and late Lyme borreliosis, especially for diagnosing erythema migrans. The methods according to the invention are characterized in that recombinant BBK32 proteins, optionally other immunogenic borrelial proteins, or their fragments are used as antigens.

La présente invention concerne une méthode destinée à détecter une infection par Borrelia burgdorferi sensu lato ou la présence d'anticorps dirigés contre les espèces Borrelia dans un liquide corporel prélevé chez un humain infecté ou vacciné suspect ou un autre mammifère. L'invention concerne également une trousse de diagnostic associée, ainsi qu'une méthode d'immunoanalyse destinée au diagnostic d'une borréliose de Lyme aux stades précoce et avancé, et notamment au diagnostic d'un érythème migrant. Les méthodes de l'invention se caractérisent en ce qu'on utilise des protéines BBK32 recombinantes, voire éventuellement d'autres protéines de Borrelia immunogènes, ou leurs fragments comme antigènes.

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