Method for diagnosing pathology in biological samples

G - Physics – 01 – N

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G01N 21/35 (2006.01) G01N 33/487 (2006.01) G01N 33/574 (2006.01) G01N 21/49 (2006.01)

Patent

CA 2428129

A method and system for diagnosing pathology, such as carcinoma, in a biological sample identifies presence of pathology based on the existence of an infrared markers in the extracellular material, rather than cells, in the biological sample. In the case of breast cancer diagnosis, an effective marker is a baseline slope of a 1280 cm-1 band in the infrared spectra of connective tissue, with normal biopsy samples exhibiting a positive slope and cancerous samples showing a relatively flat baseline. Infrared spectroscopy, both microscopic and macroscopic, may be used to identify a sample region containing extracellular material and to collect infrared absorbance data, from which the existence of the pathology marker is determined.

L'invention concerne un procédé et un système permettant de diagnostiquer une pathologie comme le carcinome. On identifie, dans un échantillon biologique, la présence d'une pathologie basée sur l'existence de marqueurs infrarouges dans le matériau extracellulaire plutôt que dans les cellules. En cas de diagnostic du cancer du sein, un marqueur efficace est une courbe de départ d'une bande de 1280 cm-1 dans le spectre infrarouge de tissu de connexion, avec des échantillons de biopsie normaux présentant une courbe positive et des échantillons cancéreux présentant un départ relativement plat. Un examen spectroscopique infrarouge, à la fois microscopique et macroscopique peut permettre d'identifier une zone d'échantillon contenant un matériau extracellulaire et de collecter des données d'absorption infrarouge, à partir desquelles l'existence du marqueur de pathologie est déterminée.

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