Method for differentiating the source of occult...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/28 (2006.01) G01N 33/72 (2006.01) A61B 10/00 (2006.01)

Patent

CA 2158432

The presence of fecal occult blood in a stool sample is detected by mixing a liquid stool sample with an acidic liquid, such as a phosphate/citrate buffer, to precipitate hematin from the solution. The precipitated hematin is separated and the presence of absence of hemoglobin is determined by exposing the solution to a peroxidase diagnostic assay. A positive response indicates the presence of blood originating in the lower gastrointestinal tract, a leading indicator of lower GI cancer.

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