Method for differentiation of stem cells

Details Method for differentiation of stem cells Method for differentiation of stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/0775 (2010.01) A61K 35/12 (2006.01)

Patent

CA 2599871

A method for the differentiation of mammalian pluripotent stem (PS) cells into a mortal multi-lineage progenitor cell population is provided which comprises culturing the pluripotent stem cells in the presence of Hyaluronan (HA). The mortal multi- lineage progenitor cell population may be a population of mesenchymal stem cells. The mortal multi-lineage progenitor cell population may form cells of the mesodermal lineage, suitably osteoblasts. Alternatively, the mortal multi-lineage progenitor cell population may form cells of the endodermal lineage or of the ectodermal lineage, which may be neuronal progenitors.

L~invention concerne un procédé de différenciation de cellules souches pluripotentes (PS) mammifères en une population de cellules parentes à multidescendance mortelles, consistant à mettre en culture les cellules souches pluripotentes en présence de Hyaluronan (HA). La population de cellules parentes à multidescendance mortelles peut être une population de cellules souches mésenchymes. La population de cellules parentes à multidescendance mortelles peut former des cellules de la descendance mésodermique, de préférence des ostéoblastes. En variante, la population de cellules parentes à multidescendance mortelles peut constituer des cellules de descendance endodermique ou de descendance ectodermique, qui peuvent être des parents neuronaux.

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