Method for discriminating between unstained and absorbing...

Details Method for discriminating between unstained and absorbing... Method for discriminating between unstained and absorbing...

G - Physics

01

N

150/13, 167/39

G01N 21/47 (2006.01) C12Q 1/02 (2006.01) G01N 15/14 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1202868

-12- METHOD FOR DISCRIMINATING BETWEEN UNSTAINED AND ABSORBING DYE STAINED CELLS ABSTRACT Method for discriminating unstained cells from stained cells in a heterogeneous population. Specified cell types are stained with an absorbing stain and all cells are passed through the class of flow cytometry instrumentation employing focused collimated light sources. Detection of low angle and wide angle light scatter permits differenti- ation between the cell types on the basis that cells stained with an absorbing stain produce compara- tively less low angle light scatter and comparatively more wide angle light scatter than do unstained cells.

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