Method for discriminating red blood cells from white blood...

G - Physics – 01 – N

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G01N 15/10 (2006.01) G01N 33/49 (2006.01) G01N 35/00 (2006.01) G06M 11/00 (2006.01)

Patent

CA 2759392

A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

L'invention concerne un procédé permettant d'identifier, d'analyser, et de quantifier les composants cellulaires du sang complet au moyen d'un analyseur hématologique automatisé, et la détection de la lumière diffusée, absorbée, et émise par fluorescence par chaque cellule. Plus précisément, ledit procédé implique d'identifier, d'analyser, et de quantifier les composants cellulaires du sang complet au moyen d'une source lumineuse dont la longueur d'onde est comprise entre environ 400 nm et environ 450 nm et de plusieurs mesures optiques de flux entrant et coloration sans besoin de lyser les globules rouges.

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