Method for electrochemically detecting nucleic acid-oligomer...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) G01N 27/06 (2006.01) G01N 27/30 (2006.01) G01N 27/327 (2006.01) G01N 27/416 (2006.01) G01N 33/566 (2006.01) G01N 37/00 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2371938

The invention relates to a method for electrochemically detecting sequence- specific nucleic acid-oligomer hybridisation events. DNA/RNA/PNA oligomer single strands which are bound to a conductive surface at one end and linked to a redoxactive unit at the other, free end, serve as a hybridisation matrix (probe). A proportion of the single strand oligonucleotides are hybridised by treatment with the oligonucleotide solution (target) being tested, with the result that the electrical communication between the conductive surface and the redoxactive unit, which is initially non- or barely existent, is increased. This enables a hybridisation event to be detected using electrochemical methods such as voltammetry, amperometry or conductance measurement.

La présente invention concerne un procédé de détection électrochimique d'hybridation d'acide nucléique-oligomère à séquence spécifique. Dans ce procédé, les brins simples d'ADN/ARN/APN-oligomères servent de matrice d'hybridation (sonde), une de leurs extrémités étant reliée à une surface conductrice, et l'autre, libre, étant connectée à une unité à action redox. Par traitement avec la solution d'oligonucléotides à analyser (cible), on hybride une partie des oligonucléotides à brin simple, augmentant ainsi la transmission électrique, nulle ou seulement faible au départ, entre la surface conductrice et l'unité à action redox. On peut ainsi détecter une hybridation par des procédés électrochimiques comme la voltamétrie, l'ampérométrie ou la conductométrie.

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