Method for electroporation and buffer used therefor

Details Method for electroporation and buffer used therefor Method for electroporation and buffer used therefor

C - Chemistry, Metallurgy

12

N

195/1.35

C12N 13/00 (2006.01) C12N 5/10 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2023884

ABSTRACT OF THE DISCLOSURE Disclosed is a method for electroporation with which higher transformation efficiency may be attained than that attained by the conventional methods. In the method of electroporation, cells and DNAs are suspended in a buffer containing potassium ion as a cation and an amino acid ion and/or an organic acid ion as an anion, and substantially not containing chloride ion, and high voltage is applied thereacross.

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