Method for endothelial cell extraction from adipose tissues

Details Method for endothelial cell extraction from adipose tissues Method for endothelial cell extraction from adipose tissues

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2644115

Adipose tissue has proven to serve as an abundant, accessible, and rich source of endothelial or vascular endothelial cells suitable for tissue engineering. We describe a detailed method for the isolation and purification of endothelial cells using purified enzymes and antibody-based selection. The cells can be obtained from liposuction procedures and used in vascular grafts.

La présente invention concerne un tissu adipeux qui sert de source abondante, accessible et riche de cellules endothéliales ou vasculaires convenant pour l'ingénierie des tissus. Nous décrivons un procédé détaillé d'isolement et de purification de cellules endothéliales en utilisant des enzymes purifiées et une sélection à base d'anticorps. Les cellules peuvent être obtenues à partir de procédures de liposuccion et utilisées lors de greffes vasculaires.

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