Method for enhanced protein stabilization and production of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/70 (2006.01) C07H 21/02 (2006.01) C07K 14/47 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12P 19/34 (2006.01) C12P 21/04 (2006.01) C12P 21/06 (2006.01) H01J 5/48 (2006.01) H01J 5/50 (2006.01) H01J 29/10 (2006.01)

Patent

CA 2372243

A method for increasing production yield of viruses, viral proteins, and other related biological materials through enhanced control and stabilization of protein production via stress proteins and the resultant protein products. The present invention is also directed to methods for selection or engineering of cell lines yielding such enhanced stabilized products. More specifically, example embodiments of the present invention are directed to methods for enhancing production of a viral agent, production of cell lines exhibiting permanent genetic modification, production of permissive eucaryotic cell lines, enhancing functional recombinant product yield, and the products of such methods.

Cette invention se rapporte à un procédé servant à accroître le rendement de virus, de protéines virales et d'autres matériaux biologiques apparentés grâce à l'amélioration du contrôle et de la stabilisation de la production de protéines via des protéines du stress et aux produits protéinés qui en résultent. Cette invention se rapporte également à des procédés servant à sélectionner ou à modifier génétiquement des lignées cellulaires permettant de fabriquer des produits stabilisés ainsi améliorés. Comme exemples de modes de réalisation, cette invention propose plus spécifiquement des procédés servant à accroître la production d'un agent viral, la production de lignées cellulaires présentant des modifications génétiques permanentes et la production de lignées cellulaires eucaryotes permissives et à améliorer le rendement de produits de recombinaison fonctionnels ainsi que les produits de ces procédés.

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