Method for enhancing expression of a foreign or endogenous...

Details Method for enhancing expression of a foreign or endogenous... Method for enhancing expression of a foreign or endogenous...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 14/08 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2285026

The present invention provides a method for enhancing the expression of genes in plants by supplying a virally encoded booster sequence comprising the 5' proximal region of the potyvirus genome to the plant. The booster sequence enhances the expression of foreign genes or endogenous plant genes in plants by employing any known methodology of expressing introduced genes in plants. The booster sequence may be used to enhance expression of any gene, including foreign genes or endogenous plant genes, introduced by means of stable transformation into the genome of the plant or introduced by expression from a plant viral expression vector.

Ce procédé, qui permet de renforcer l'expression génique chez des végétaux, consiste à apporter au végétal une séquence d'appoint viralement codée comportant la région 5' proximale du génome de potyvirus. Cette séquence d'appoint permet de renforcer l'expression des gènes étrangers ou de gènes végétaux endogènes chez des végétaux dès lors qu'on a recours à l'une quelconque des techniques d'expression applicables aux gènes introduits dans les végétaux. Cette séquence d'appoint convient au renforcement de l'expression de n'importe quel gène, y compris des gènes étrangers ou des gènes de végétaux endogènes, introduit par transformation stable en génome du végétal ou introduit par expression à partir d'un facteur d'expression virale de végétal.

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