Method for enhancing gene transfer

Details Method for enhancing gene transfer Method for enhancing gene transfer

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 31/17 (2006.01) A61K 31/70 (2006.01) A61K 35/28 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2257880

The present invention provides a method for optimising gene transfer into hematopoietic stem cells (HSCs) by contacting the cells with hydroxyurea prior to gene transfer to induce HSCs in G0 into active cell cycle. This method is useful for treating patients suffering from a disease that is suitably treated by gene therapy or involves hematopoietic cells. A method is also provided for enhancing the efficacy of bone marrow transplantation by administering hydroxyurea to increase HSC yields.

L'invention concerne une méthode qui permet d'optimiser le transfert de gènes dans les cellules souches hématopoiétiques multipotentes (CFU-S) en mettant lesdites cellules, avant le transfert, en contact avec de l'hydroxyurée, de façon à pousser les cellules en phase 0 à passer dans le cycle cellulaire actif. Cette méthode est utile pour traiter des patients atteints d'une maladie pouvant être convenablement traitée par thérapie génique ou mettant en jeu les cellules hématopoiétiques. L'invention concerne également une méthode qui permet d'accroître l'efficacité des greffes de moelle osseuse en administrant de l'hydroxyurée, ce qui accroît la formation de CFU-S.

Affiliated with

Also associated with

Rating

Method for enhancing gene transfer does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for enhancing gene transfer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for enhancing gene transfer will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1839444