C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/22 (2006.01)
Patent
CA 2689305
A method for enhancing the cleavage activity of an I-CreI derived meganuclease, comprising the site-specific mutation of at least one amino acid residue which is selected in the group consisting of: the glycine at position 19, the phenylalanine at position 54, the phenylalanine at position 87, the serine at position 79, the valine at position 105 and the isoleucine at position 132 of l-Crel, and its application for the manufacturing of meganuclease cleaving a DNA target of interest, for use in genome therapy (treatment of genetic diseases) and genome engineering (making of transgenic animals, transgenic plants and recombinant cell lines).
L'invention porte sur un procédé permettant d'améliorer l'activité de clivage d'une méganucléase issue de I-CreI. Ce procédé comprend la mutation spécifique d'un site d'au moins un résidu d'acide aminé qui est choisi parmi le groupe constitué par: la glycine en position 19, la phénylalanine en position 54, la phénylalanine en position 87, la sérine en position 79, la valine en position 105 et l'isoleucine en position 132 de l-Crel. L'invention porte également sur l'application de ce procédé pour la fabrication d'une méganucléase clivant une cible d'ADN d'intérêt, pour une utilisation en thérapie génomique (traitement de maladies génétiques) et en ingénierie génomique (création d'animaux transgéniques, de plantes transgéniques et de lignes cellulaires recombinantes).
Gouble Agnes
Grizot Sylvestre
Perez-Michaut Christophe
Cellectis
Robic
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