Method for enhancing the translation and expression of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07K 1/00 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/54 (2006.01) C07K 16/00 (2006.01) C12N 1/20 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12N 15/75 (2006.01) C12N 15/76 (2006.01) C12P 19/34 (2006.01) C12P 21/00 (2006.01) C12P 21/04 (2006.01) C12P 21/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2444298

The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in prokaryotic cells. A strategy is provided for enhancing the synthesis of recombinant amino acid sequences by improving translation from expression cassettes in vitro before producing recombinant hosts.

Selon l'invention, l'utilisation accrue de techniques d'exploration de données concernant des séquences nucléotidiques a amplifié la demande de procédés plus efficaces de production de protéines recombinées dans des cellules procaryotes. L'invention concerne une stratégie permettant de renforcer la synthèse de séquences d'acides aminés recombinées par amélioration de la traduction de cassettes d'expression in vitro avant de produire des hôtes recombinés.

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