C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2552010
The invention relates to an improved method for enriching and stabilising components which contain DNA and which are made of biological materials, in particular, blood samples. The sample materials containing DNA are partially lysed in a lysis/bonding puffer system and the components containing DNA, such as cell nuclei, are bound to a functionalised solid surface. Said system comprises lysis reagents and solid adsorbents. The surface of the adsorbents are functionalised with polymers from polymerisable acids or derivatives thereof, whereon the components containing DNA bind. Organic or inorganic solid materials act as carrier materials which can be functionalised.
L'invention concerne une amélioration apportée à un procédé pour enrichir et stabiliser des constituants, contenant de l'ADN, de matières biologiques, notamment d'échantillons sanguins. Les matières d'échantillons, contenant de l'ADN, sont partiellement lysées dans un système tampon de lyse-liaison et les constituants, contenant de l'ADN, par exemple des noyaux cellulaires, sont liés à une surface solide fonctionnalisée. Ce système comprend des réactifs de lyse et des adsorbants solides. Les surfaces de ces derniers sont fonctionnalisées avec des polymères d'acides polymérisables ou de leurs dérivés, auxquels se lient les constituants contenant de l'ADN. Des matières solides organiques ou inorganiques peuvent servir de matières de support fonctionnalisables.
Schubert Frank
Wambutt Rolf
Agowa Gesellschaft Fur Molekularbiologische Technologie Mbh
Teitelbaum & Maclean
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