Method for enzymatic preparation of homogentisate

Details Method for enzymatic preparation of homogentisate Method for enzymatic preparation of homogentisate

C - Chemistry, Metallurgy

12

P

C12P 7/42 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2315903

The invention concerns a method for enzymatic preparation of homogentisate (HMO) from 4-hydroxypyruvate (HPP), characterised in that it consists in carrying out in a suitable reaction medium the following enzymatic reactions: enzymatic conversion of HPP into 4-hydroxyphenylacetate (HPA) with a first appropriate enzyme; then an enzymatic conversion of HPA into HMO with a second appropriate enzyme.

La présente invention concerne un procédé de préparation enzymatique d'homogentisate (HMO) à partir de 4-hydroxypyruvate (HPP), caractérisé en ce qu'il consiste à effectuer dans un milieu réactionnel approprié les réactions enzymatiques suivantes: conversion enzymatique du HPP en 4- hydroxyphénylacétate (HPA) par une première enzyme appropriée, puis, conversion enzymatique de HPA en HMO par une deuxième enzyme appropriée.

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