Method for enzyme immunoassay of progesterone

G - Physics – 01 – N

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167/38

G01N 33/74 (2006.01)

Patent

CA 1071535

ABSTRACT OF THE DISCLOSURE " A method for enzyme immunoassay of progesterone " A method for enzyme immunoassay of progesterone. The method for enzyme immunoassay of progesterone making use of competitive reactions in a liquid medium of molecules of the said progesterone, molecules referred to as Ag, and molecules of progesterone coupled to an enzyme, namely .beta.-D-galactosidase (3.2.1.23), molecules referred to as Ag-GZ, for the same sites of an antibody consisting of an anti-progesterone serum referred to as Ab1 and of precipitation of soluble complexes obtained by a second insoluble antibody referred to as Ab2, the reaction resulting in a solid phase containing all the antibody populations and of a supernatent containing the Ag and Ag-GZ fractions which have not reacted with Ab1, is characterized by the use of a same progesterone derivative, namely progesterone -11-.alpha.-hemisuccinate, for coupling with .beta.-galactosidase (3.2.1.23) and for the production of anti-progesterone serum. Determination of progesterone in plasma.

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