Method for evaluating cell populations

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) C12N 5/078 (2010.01) C07H 21/00 (2006.01) C12N 9/10 (2006.01) C12Q 1/04 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2652767

The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.

L'invention concerne des structures sialylées spécifiques présentes sur des cellules souches humaines et des populations cellulaires dérivées de celles-ci. L'invention concerne plus précisément des méthodes permettant de contrôler l'état de cellules souches, consistant à observer des changement au niveau de la sialylation des cellules ; et à contrôler les contaminations potentielles du matériel biologique. L'invention concerne également des réactifs et des méthodes utilisés avec ces cellules pour éviter des altérations au niveau de la glycosylation des cellules par des contaminants. L'invention concerne également des nouvelles cellules souches dont la glycosylation a été altérée de manière spécifique.

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