Method for evaluating compound using barlp and substance for...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) A61K 45/00 (2006.01) A61P 3/04 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2606083

It is intended to provide a method for evaluating a compound which regulates eating or body weight characterized by comprising the steps of introducing a BARLP gene and preparing a cell expressing BARLP; bringing a test compound into contact with the cell; and detecting a specific binding of the test compound to the BARLP and a method for evaluating a compound further comprising the step of evaluating a test compound using a nonhuman genetically- engineered animal in which the BARLP gene is inactivated. According to this invention, knowledge about a relationship between BARLP and biological functions is obtained and a method for evaluating a compound targeting BARLP and a BARLP ligand obtained by the evaluation can be provided.

L~invention concerne un procédé permettant d~évaluer un composé qui régule la faim ou le poids corporel Cette méthode se caractérise par les étapes suivantes : l~introduction d~un gène BARLP et la préparation d~une cellule exprimant le BARLP ; la mise en contact d~un composé test avec la cellule ; et la détection d~une liaison spécifique du composé test au BARLP. L~invention concerne également un procédé permettant d~évaluer un composé, qui comprend aussi l~étape consistant à évaluer un composé test à l~aide d~un animal non humain génétiquement modifié dans lequel le gène BARLP a été inactivé. Cette invention permet d~obtenir des informations sur le lien existant entre le BARLP et des fonctions biologiques, un procédé permettant d~évaluer un composé ciblant le BARLP, ainsi qu~un ligand BARLP obtenu à la suite de l~évaluation.

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