Method for evaluating oil-and-fat amount in seed and method...

Details Method for evaluating oil-and-fat amount in seed and method... Method for evaluating oil-and-fat amount in seed and method...

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) C12N 15/09 (2006.01) G01N 33/00 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2717067

This invention is intended to evaluate the oil-and-fat amount in a seed and genetic changes thereof: A fusion protein of an oil body-specific protein, such as the oleosin-GFP fusion protein, and a fluorescent protein is expressed, and the oil-and-fat amount in a plant seed and changes thereof are evaluated based on the fluorescent intensity in a cotyledon of a fluorescent protein, such as GFP.

L'invention concerne l'évaluation de la quantité de graisse et d'huile dans une graine et de la modification génétique de celle-ci. La quantité de graisse et d'huile contenue dans une graine d'un corps végétal et la modification de celle-ci sont déterminées à partir de l'intensité de fluorescence d'une protéine fluorescente, par exemple une GFP, dans un cotylédon par expression d'une protéine de fusion faite d'une protéine spécifique à un corps huileux et d'une protéine fluorescente, par exemple une protéine de fusion oléosine-GFP.

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