G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01) G01N 33/04 (2006.01)
Patent
CA 2264674
The invention concerns a method for evaluating the hot treatment to which a proteinic nutrient such as milk is subjected. It consists in the following steps: a) taking a sample of said nutrient and adding thereto an ionic strength pad and appropriate pH for obtaining a precipitate of proteins denatured by the hot treatment and a transparent supernatant containing proteins which are still soluble; b) analysing the tryptophan present in the supernatant; and c) analysing the fluorescent by-products derived from the advanced Maillard reaction present in the supernatant. The invention is useful in methods for hot treatment of proteinic nutrients.
Méthode pour évaluer le traitement thermique auquel est soumis un aliment protéique tel qu'un lait. Elle comprend les étapes suivantes: a) on prélève un échantillon dudit aliment auquel on ajoute un tampon de force ionique et de pH appropriés pour obtenir, d'une part, un précipité de protéines dénaturées par le traitement thermique et, d'autre part, un sumageant transparent qui renferme des protéines encore solubles; b) on dose le tryptophane présent dans le surnageant; et c) on dose les sous-produits fluorescents issus de la réaction avancée de Maillard présents dans le surnageant. Application à la conduite des procédés de traitement thermique d'aliments protéiques.
Institut National Agronomique Paris-Grignon
Robic
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