Method for evaluating the virulence of pathogenic biphasic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2732305

A method for evaluating relative bacterial virulence of a biphasic bacteria in environmental systems includes measuring the concentration of DNA in the bacteria, measuring the concentration of RNA in the bacteria, determining a ratio of the concentration of RNA to the concentration of DNA and correlating the concentration ratio with a level of relative pathogenicity, wherein the bacteria is preferentially Legionella pneumophila, Mycobacterium tuberculosis and Listeria.

L'invention concerne un procédé pour évaluer une virulence bactérienne relative d'une bactérie biphasique dans des systèmes environnementaux, comprenant la mesure de la concentration d'ADN par la bactérie, la mesure de la concentration d'ARN dans la bactérie, la détermination d'un rapport de la concentration d'ARN sur la concentration d'ADN, et la mise en corrélation du rapport de concentration avec un niveau de pathogénicité relative, la bactérie étant de préférence Legionella pneumophila, Mycobacterium tuberculosis et Listeria.

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